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Surface marker-dependent isolation procedures are considered to be highly efficient, less time-consuming, and moreover easily translatable to therapeutic applications. Aside from a subtype-specific genetic modification, none of these methods facilitate the selective enrichment of atrial or ventricular CMs. Several purification methods have been described to enrich CMs, such as physical separation, fluorescent reporter or antibiotic resistance genes, molecular beacons, labeling with mitochondrial dyes, or metabolic selection. Due to the lack of appropriate isolation strategies, the characterization and use of CM subtypes is limited. As they differ in origin, electrophysiological properties and gene expression, they are of special interest in heart research and regenerative medicine. The four-chambered mammalian heart comprises different muscle cell populations, for example chamber-specific atrial and ventricular cardiomyocytes (CMs). This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.

were employed by Miltenyi Biotec GmbH, Germany, which has filed a patent application based on this study. The specific role of these authors is articulated in the 'author contributions' section.Ĭompeting interests: A.M.W., M.K., J.E., S.T., P.C., A.B. Miltenyi Biotec GmbH provided support in the form of salaries for authors, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

was supported by a scholarship from “Science Without Borders” program of CNPq-Brazil he performed all the experimental work during his stay at the Institute of Physiology I, Life & Brain Center, University of Bonn.
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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedĭata Availability: The mRNA data discussed in this publication has been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE57131 ( ).įunding: G.M. Received: Accepted: NovemPublished: November 30, 2015Ĭopyright: © 2015 Wiencierz et al. PLoS ONE 10(11):Įditor: Enzo Porrello, The University of Queensland, AUSTRALIA (2015) Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.
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You are solely responsible for adequate protection and backup of the data and equipment used in connection with using software LabChart Reader.Citation: Wiencierz AM, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, et al. The use of the software and any damage done to your systems. PCWin has not developed this software LabChart Reader and in no way responsible for
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